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Image Search Results
Journal: Cell metabolism
Article Title: Digoxin Suppresses Pyruvate Kinase M2 Promoted HIF-1α Transactivation in Steatohepatitis
doi: 10.1016/j.cmet.2018.01.007
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PKM2 (D78A4) Cell Signaling Technology Cat#:4053; BRID: AB_1904096 Histone H3 Cell Signaling Technology Cat #:2650; BRID: AB_2115124 IL-1beta R & D Cat#:AF401-NA; BRID:AB_416684 Caspase-1p10 (M20) Santa Cruz Biotechnology Cat#: SC-514; BRID: AB_2068895 NLRP3 (Cyro-2) AdipoGen Life Sciences Cat#: AG-20B-0014; BRID: AB 2490202 Ly6C (CL21)-FITC BD Biosciences Cat#: 553104; BRID: AB_394628 Ly6G (1A8)-PE BD Biosciences Cat#:551461; BRID: AB_394208 Chemicals, Peptides, and Recombinant Proteins Digoxin Sigma D6003 2-mercaptoethanol Thermo Fisher Scientific 21985-023 diisocyanato hexane Sigma 52649 M-CSF PeproTech 315-02 methylamine Sigma 426466 Lipofectamine™ 2000 Thermo Fisher Scientific 11668027 D-galactosamine Calbiochem CAS-1772-03-08 Critical Commercial Assays PolymorphPrep AXIS-SHIELD DENSITY GRADIENT MEDIA AXS-1114683 TentaGel NH2 Resin Advanced Chem Tech SJ6011 Nuclear Extraction Kit EMD Millipore Corporation 2900 2x SYBR Green I Master Roche Cat#: 04707516001 Deposited Data Gene microarray of HFD liver tissue GSE108839 Experimental Models: Cell Lines Human: THP-1 cells ATCC TIB-202 Human: HEK293 T cells ATCC CRL-11268 Mouse: Raw264.7 ATCC TIB-71 Experimental Models: Organisms/Strains Lysozyme-Cre mouse Jackson Laboratories #004781 C57BI/6 National Cancer Institute C57BI/6 Software and
Techniques: Recombinant, SYBR Green Assay, Microarray, Software
Journal: Cell metabolism
Article Title: Digoxin Suppresses Pyruvate Kinase M2 Promoted HIF-1α Transactivation in Steatohepatitis
doi: 10.1016/j.cmet.2018.01.007
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PKM2 (D78A4) Cell Signaling Technology Cat#:4053; BRID: AB_1904096 Histone H3 Cell Signaling Technology Cat #:2650; BRID: AB_2115124 IL-1beta R & D Cat#:AF401-NA; BRID:AB_416684 Caspase-1p10 (M20) Santa Cruz Biotechnology Cat#: SC-514; BRID: AB_2068895 NLRP3 (Cyro-2) AdipoGen Life Sciences Cat#: AG-20B-0014; BRID: AB 2490202 Ly6C (CL21)-FITC BD Biosciences Cat#: 553104; BRID:
Techniques: Recombinant, SYBR Green Assay, Microarray, Software
Journal: Cell metabolism
Article Title: Digoxin Suppresses Pyruvate Kinase M2 Promoted HIF-1α Transactivation in Steatohepatitis
doi: 10.1016/j.cmet.2018.01.007
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PKM2 (D78A4) Cell Signaling Technology Cat#:4053; BRID: AB_1904096 Histone H3 Cell Signaling Technology Cat #:2650; BRID: AB_2115124 IL-1beta R & D Cat#:AF401-NA; BRID:AB_416684 Caspase-1p10 (M20) Santa Cruz Biotechnology Cat#: SC-514; BRID: AB_2068895 NLRP3 (Cyro-2) AdipoGen Life Sciences Cat#: AG-20B-0014; BRID:
Techniques: Recombinant, SYBR Green Assay, Microarray, Software
Journal: Cell metabolism
Article Title: Digoxin Suppresses Pyruvate Kinase M2 Promoted HIF-1α Transactivation in Steatohepatitis
doi: 10.1016/j.cmet.2018.01.007
Figure Lengend Snippet: (A) Whole cell lysates from LPS stimulated Raw264.7 cells were applied on digoxin-immobilized affinity resin (upper). LC-Mass spectrum (LC-MS/MS) of digoxin bound proteins identified PKM2 (middle), and the detected peptide sequence of PKM2 was identical and shown as red (bottom).
Article Snippet: REAGENT or
Techniques: Liquid Chromatography with Mass Spectroscopy, Sequencing
Journal: Cell metabolism
Article Title: Digoxin Suppresses Pyruvate Kinase M2 Promoted HIF-1α Transactivation in Steatohepatitis
doi: 10.1016/j.cmet.2018.01.007
Figure Lengend Snippet: (A) Nuclear protein lysates from LPS stimulated Raw264.7 cells were immune-precipitated using anti-PKM2 antibody. PKM2 bound proteins were identified by LC-MS/MS. The PKM2 bound histones were extracted as A.
Article Snippet: REAGENT or
Techniques: Liquid Chromatography with Mass Spectroscopy
Journal: Cell metabolism
Article Title: Digoxin Suppresses Pyruvate Kinase M2 Promoted HIF-1α Transactivation in Steatohepatitis
doi: 10.1016/j.cmet.2018.01.007
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or
Techniques: Recombinant, Extraction, SYBR Green Assay, Microarray, Software